Disney and Warner Bros. both have duck characters - Donald and Daffy - who both have bad tempers, both have constant bad luck, both are second banana to the companies main stars (with Daffy, he's second banana to both Porky - who was the star when he was created, though it'd reverse after Porky was no longer the star - and Bugs), and both arguably more popular than the face of the company (of course Donald Duck was more interesting than Mickey Mouse, you could argue that Daffy's more interesting than Porky but Bugs Bunny was allowed a lot more personality than Mickey). Daffy Duck kinda helped the Looney Tunes gain the identity it would go on to be known by, though I don't know if Donald's introduction changed anything for Disney.
Of course, when you look at the three main stars of both animated franchises - Mickey, Donald, and Goofy for Disney, Bugs, Daffy, and Porky for WB, they don't entirely parallels, since Porky rarely appears with Bugs but Daffy regularly co-stars with both characters. In fact, you could argue that Bugs, Daffy, and Elmer are more of a trio in this regard, even though Elmer Fudd is a clear bad guy (even if it's often more designated) while there is no villain in the Mickey/Donald/Goofy trio, but then again, those three are buddies and in many shorts where they appear as a trio, they tend to separate and get their own solo scenes for much of the shorts, while Bugs, Daffy, and Elmer don't really separate, but they're also pretty much all enemies.
Of course, most of the classic Disney animated characters are friends/a team, while many of the Looney Tunes characters are separate/at odds with each other. In thinking about this, it seems Warner Bros. has a lot more main characters, about 12 (not counting Bosko or Buddy), while with Disney it's.... also 12, but more of the Disney characters are duos/trios rarely seen without certain characters, while more of the main Looney Tunes characters can appear frequently without certain characters. And even then, Disney didn't have many one-shot characters outside of the Silly Symphonies series and not many minor characters who star without the main cast, while Looney Tunes had a lot more one-shots and a lot more minor characters independent of any star characters.